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Inorganic flame retardants and their application in composites
Bergmann, Ondřej ; Kučera, František (referee) ; Poláček, Petr (advisor)
In the first part of this bachelor thesis frequently used fire retardant additives are described as well as their mechanisms of action and the most common methods of flammability characterization. In the second experimental part the influence of two systems, consisting of a matrix and two fillers in different ratios, on viscosity, thermal stability and flammability were examined. Unsaturated polyester resin was used as the matrix. The first filler was always a fire retardant based on aluminium hydroxide, which was combined with the second filler based on either ammonium polyphosphate, or melamine polyphosphate. Viscosity of these samples was determined and casts for further analyses were made. Casts were analysed with thermogravimetric analysis and by flammability tests. Generally, with higher filler content the samples were more viscous, thermally degraded at lower temperatures and had better fire characteristics.
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Inorganic flame retardants and their application in composites
Bergmann, Ondřej ; Kučera, František (referee) ; Poláček, Petr (advisor)
In the first part of this bachelor thesis frequently used fire retardant additives are described as well as their mechanisms of action and the most common methods of flammability characterization. In the second experimental part the influence of two systems, consisting of a matrix and two fillers in different ratios, on viscosity, thermal stability and flammability were examined. Unsaturated polyester resin was used as the matrix. The first filler was always a fire retardant based on aluminium hydroxide, which was combined with the second filler based on either ammonium polyphosphate, or melamine polyphosphate. Viscosity of these samples was determined and casts for further analyses were made. Casts were analysed with thermogravimetric analysis and by flammability tests. Generally, with higher filler content the samples were more viscous, thermally degraded at lower temperatures and had better fire characteristics.
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Revealing phosphoproteins playing role in tobacco pollen activated in vitro
Fíla, Jan ; Honys, David (advisor) ; Fischer, Lukáš (referee)
5 Abstract Tobacco mature pollen rehydrates in vivo on a stigma tissue, and develops into the rapidly-growing pollen tube. This rehydration process is accompanied by the de-repression of stored mRNA transcripts, resulting in the synthesis of novel proteins. Furthermore, such metabolic switch is also likely to be regulated on the level of post-translational modifications of the already-present proteins, namely via phosphorylation, since it was shown to play a significant regulatory role in numerous cellular processes. Since only a minor part of proteins is phosphorylated in a cell at a time, the employment of various enrichment techniques is usually of key importance. In this diploma project, metal oxide/hydroxide affinity chromatography (MOAC) with aluminium hydroxide matrix was applied in order to enrich phosphoproteins from the mature pollen and the 30-minute in vitro activated pollen crude protein extracts. The enriched fraction was separated by both 2D-GE and gel-free liquid chromatography (LC) approaches with subsequent mass spectrometric analyses. Collectively, 139 phosphoprotein candidates were identified. Additionally, to broaden the number of phosphorylation sites identified, titanium dioxide phosphopeptide enrichment of trypsin-digested mature pollen crude extract was performed. Thanks to the...
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Revealing phosphoproteins playing role in tobacco pollen activated in vitro
Fíla, Jan ; Honys, David (advisor) ; Fischer, Lukáš (referee)
5 Abstract Tobacco mature pollen rehydrates in vivo on a stigma tissue, and develops into the rapidly-growing pollen tube. This rehydration process is accompanied by the de-repression of stored mRNA transcripts, resulting in the synthesis of novel proteins. Furthermore, such metabolic switch is also likely to be regulated on the level of post-translational modifications of the already-present proteins, namely via phosphorylation, since it was shown to play a significant regulatory role in numerous cellular processes. Since only a minor part of proteins is phosphorylated in a cell at a time, the employment of various enrichment techniques is usually of key importance. In this diploma project, metal oxide/hydroxide affinity chromatography (MOAC) with aluminium hydroxide matrix was applied in order to enrich phosphoproteins from the mature pollen and the 30-minute in vitro activated pollen crude protein extracts. The enriched fraction was separated by both 2D-GE and gel-free liquid chromatography (LC) approaches with subsequent mass spectrometric analyses. Collectively, 139 phosphoprotein candidates were identified. Additionally, to broaden the number of phosphorylation sites identified, titanium dioxide phosphopeptide enrichment of trypsin-digested mature pollen crude extract was performed. Thanks to the...
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